mouse anti atp5a  (Proteintech)

Journal: iScience

Article Title: BCL2L13 at endoplasmic reticulum-mitochondria contact sites regulates calcium homeostasis to maintain skeletal muscle function

doi: 10.1016/j.isci.2024.110510

Figure Lengend Snippet:

Article Snippet: After blocking, primary antibodies were incubated overnight at 4°C: mouse anti-MYH2 (1:500, A4.74, Santa Cruz Biotechnology), mouse anti-ATP5a (1:500, ab14748, Abcam, Waltham, MA, United States), mouse anti-PDHα (1:1000, ab110333, Abcam), rabbit anti-PDH E1α phospho-serine 293 (1:1000, ab177461, Abcam), rabbit anti-BCL2L13 (1:500, N-ter, HPA050377, Sigma), rabbit anti-BCL2L13 (1:500, center, HPA030994, Sigma), mouse anti-FACL4 (1:500, ab155282, Abcam), mouse anti-NDUFS3 (1:1000, ab14711, Abcam), mouse anti-SERCA2 (1:500, ab150435, Abcam), rabbit anti-RyR1 antibody (Marks’ lab, Columbia University, NY, USA, Cat. #: 5,029, Aa 1327–1339), rabbit anti-MCU (1:1000, HPA016480, Sigma), mouse anti-SERCA1 (1:1000, ab2819, Abcam), mouse anti-αTubulin (1:2000, T6199, Sigma), mouse anti-IP3R-I/II/III (1:200, sc-377518, Santa Cruz biotechnology) and rabbit anti-cleaved Caspase 3 (1:500, 9661S, Cell signaling, Danvers, United States).

Techniques: Virus, Subcloning, Recombinant, Staining, Labeling, Gel Extraction, SYBR Green Assay, Reverse Transcription, Western Blot, esiRNA, Fluorescence In Situ Hybridization, Clone Assay, Plasmid Preparation, Software, Microscopy, Mass Spectrometry, Transmission Assay, Imaging